Search results for "Model substrates"

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Monitoring of transglutaminase crosslinking reaction by 1H NMR spectroscopy on model substrates

2015

International audience; A new method based on 1H NMR spectroscopy was developed for monitoring transglutaminase crosslinking reaction with model molecules (CBZ-Gln-Gly and N-α-acetyl-lysine). The transglutaminase reaction led to the appearance of new resonances on NMR spectrum as well as significant decrease in others. The new observed resonances, originated from newly formed ɛ-(γ-glutamyl)lysine isopeptide bonds, evidence the enzymatic reaction and allow to quantify the ɛ-(γ-glutamyl)lysine fragment. Moreover, the decrease in resonance intensity, originated from lysine, permit to determine the crosslinking degree. These results obtained by 1H NMR spectroscopy can be used as an alternative …

0106 biological sciences1h nmr spectroscopyTissue transglutaminaseLysineCrosslinking degreePhotochemistrycomplex mixtures01 natural sciences03 medical and health sciencesModel substratesɛ-(γ-glutamyl)-lysineColloid and Surface ChemistryLiquid chromatography–mass spectrometry010608 biotechnologyOrganic chemistryMolecule[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology030304 developmental biologyAlternative methods0303 health sciencesbiologyChemistryResonanceNuclear magnetic resonance spectroscopyMicrobial transglutaminasebiology.proteinColloids and Surfaces A: Physicochemical and Engineering Aspects
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